• B. Vijaya Ambica Durga
• V.N.V.K.Sravani
• S.N.L.Pravallika
• Dr.V.Bhaskara Raju

The ethanol extract of Ocimum sanctum was investigated for in vivo alcohol withdrawal effects. Ocimum sanctum L, a significant medicinal plant, affects Wistar rats experiencing alcohol withdrawal techniques. The rats were fed a liquid diet containing 7.2% ethanol by volume for a period of 21 days. Animals in the control group were given sucrose as a liquid diet that is isocaloric. Rats were observed at 6th and 24th hours post-alcohol withdrawal for indicators of severe withdrawal, such as anxiety and hyperlocomotor activity. Ethanol withdrawal anxiety was tested using an elevated plus maze, a light and dark model, and the hyperlocomotor activity was measured using an actophotometer. O. sanctum leaf extract (100, 200, and 300 mg/kg, oral) and diazepam (2 mg/kg, i.p.) were administered to the treatment group animals 30 min before alcohol withdrawal estimation. Drug treatment was also given 30 min before the second observation at the 24th hour. On the last day of the protocol, rats were sacrificed by cervical dislocation. liver, kidney, and brain were isolated and preserved in formalin for further histopathological examination.

Elevated plus maze, light and dark model, Actophotometer

"EVALUATION OF ALCOHOL WITHDRAWAL ACTIVITY OF ETHANOLIC EXTRACT OF OCIMUM SANCTUM LINN", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 12, page no.g647-g655, December-2024, Available :http://www.jetir.org/papers/JETIR2412673.pdf

"EVALUATION OF ALCOHOL WITHDRAWAL ACTIVITY OF ETHANOLIC EXTRACT OF OCIMUM SANCTUM LINN", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 12, page no. ppg647-g655, December-2024, Available at : http://www.jetir.org/papers/JETIR2412673.pdf