• Bhavika Kaklotar
• Dr. Mayuri Dholaria

Background & Objective: Bacteria tend to live in a community-like assembly called biofilm on poly matrix surfaces. Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly resistant to antimicrobial agents. There are various methods to detect biofilm production like Tube method (TM), Congo Red Agar method (CRA), Micro Titer Plate Method (MTP), bio luminescent assay, piezoelectric sensors, and fluorescent microscopic examination. Objective of This study was conducted to compare three methods for the detection of biofilms. Method: The study was carried out from May 2023 to October 2023 for checking biofilm formation capacity of respiratory tract pathogens. A total of 300 clinical isolates were subjected to biofilm detection methods. Isolates were identified biochemically by standard microbiological procedures. Antibiotic susceptibility test of isolates was performed by using the Kirby-Bauer disc diffusion technique according to CLSI guidelines. Biofilm detection was tested by TM, CRA plate method, MTP method. Results: The MTP method was considered to be superior to TM and CRA plate method. From the total of 300 MDR clinical isolates, MTP method detected 83% as high, 12.33% moderate and 4.66% as weak or non-biofilm producers. As it gives good results as compare to the TM and CRA plate method. it was also observed that higher antibiotic resistance shown in biofilm producing bacteria than non-biofilm producers. Conclusion: We can conclude from our study that the TCP method is a more quantitative and reliable method for the detection of biofilm forming microorganisms as compared to TM and CRA methods, and it can be recommended as a general screening method for detection of biofilm producing bacteria in laboratories.

MDR, biofilm, antibiotic resistance, respiratory tract infection

"Comparative Study of Different Methods for Biofilm Formation Capacity of Bacteria Causing Respiratory Tract Infection", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 1, page no.a950-a955, January-2025, Available :http://www.jetir.org/papers/JETIR2501120.pdf

"Comparative Study of Different Methods for Biofilm Formation Capacity of Bacteria Causing Respiratory Tract Infection", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 1, page no. ppa950-a955, January-2025, Available at : http://www.jetir.org/papers/JETIR2501120.pdf