• Kankan K. Maity
• Prasenjit Maity
• Dipanwita Maity
• Balaram Das

Abstract: An immunoenhancing water-soluble heteropolysaccharide (PS) with molecular weight ~1.96 × 105 Da was isolated from the hot water extract of the leaves of Azadirachta indica. Sugar analysis revealed that PS was made up of L-arabinose, L-rhamnose, D-galactose, and D-galacturonic acid. This polysaccharide exhibited splenocyte, thymocyte as well as macrophage activations. The structure of the molecule was investigated using acid hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and 1D/2D NMR studies and the repeating unit of the polysaccharide was established as: A →4)-α-D-GalpA-(1→ 2 ↑ 1 α-L-Araf-(1→6)-β-D-Galp-(1→2)-β-L-Rhap B C D

Azadirachta indica; Heteropolysaccharide; NMR Spectroscopy; Immunological study

"Structural studies of immunoenhancing heteropolysaccharide from Azadirachta indica leaves", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 1, page no.d223-d247, January-2025, Available :http://www.jetir.org/papers/JETIR2501325.pdf

"Structural studies of immunoenhancing heteropolysaccharide from Azadirachta indica leaves", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 1, page no. ppd223-d247, January-2025, Available at : http://www.jetir.org/papers/JETIR2501325.pdf