• Dr. Jayashree P
• Dr. Mathumala Subramanian
• Dr. Jaya karthik.S.S
• Dr. M. Arunachalam
• Dr. M. Sathish kumar

Access to diagnostic services is crucial for disease prevention and treatment, particularly in underprivileged populations facing resource constraints and limited healthcare infrastructure. Tissue staining techniques, such as Haematoxylin and Eosin (H&E) staining, offer cost-effective and reliable diagnostic methods that are well-suited for low-resource settings. These techniques enhance the contrast between different components of tissue samples, enabling pathologists to visualize cellular structures and abnormalities effectively staining, the most widely used histological staining method, provides high-resolution images of tissue morphology, aiding in the diagnosis of various diseases, including cancer, infections, and autoimmune disorders. The process involves fixation, dehydration, embedding, and staining, resulting in blue-stained nuclei and pink-to-red cytoplasm, which allows detailed examination under a microscope. Its applications extend to disease diagnosis, treatment monitoring, research, and education. Despite its advantages—such as simplicity, accessibility, and cost-effectiveness—H&E staining has limitations, including its qualitative nature, potential artifacts, and lack of specificity for molecular markers. Recent advances emphasize the need for automated image analysis tools to complement manual interpretation, improving diagnostic accuracy and reducing observer variability. This review highlights the importance of H&E staining in pathology and biomedical research, its procedural nuances, and its critical role in understanding tissue morphology and guiding therapeutic decisions, particularly in resource-limited environments.

Haematoxylin and Eosin stains, Cellular Morphology, Disease Mechanisms, Microscopic Analysis, Pathological Evaluation

"HEMATOXYLIN AND EOSIN STAINS: “THE ART AND SCIENCE OF TISSUE DIAGNOSTICS"- A REVIEW ARTICLE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 1, page no.g393-g402, January-2025, Available :http://www.jetir.org/papers/JETIR2501642.pdf

"HEMATOXYLIN AND EOSIN STAINS: “THE ART AND SCIENCE OF TISSUE DIAGNOSTICS"- A REVIEW ARTICLE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 1, page no. ppg393-g402, January-2025, Available at : http://www.jetir.org/papers/JETIR2501642.pdf