• Kumari Sneha

This research seeks to investigate the role of free flavonoids in anti-inflammatory activity. Punica granatum peel, also known as pomegranate is a rich reservoir of various secondary metabolites, especially flavonoids. The three parent flavonoids namely catechin, quercetin, and myricetin, were extracted from High Yield Phenolic Extract (HYPE) of Punica granatum agrowaste using an isocratic system. For bioenhancement, HYPE was subjected to a process of conversion into supramolecular phospholipid complexes and administered to rats via the peroral route. Serum samples were analyzed using a pre-developed LC-ESI-MS/MS method to improve the sensitivity of detection. At 250C column temperature, quantification was carried out using the Selected Ion Monitoring System (SIM). Gradient elution was employed and the flow rate was maintained at .5ml/min. After administering the drugs, blood samples were collected at specific time intervals of 0, 0.75, 1.25, 1.75, and 2.25 hours. The concentrations of free flavonoids from the HYPE and its phospholipid complexes were detected as 0.355, 0, and 0.525 ng/ml for catechin, myricetin, and quercetin respectively at the end of 1.75 hours. However, no further detection of these compounds was observed thereafter, indicating that they had been metabolized. Therefore, anti-inflammatory activity after 2nd hour is contributed by the metabolite of flavonoids and not their free form.

Punica granatum; flavonoids; catechin; quercetin; myricetin; phospholipid complexes; LC-MS/MS; detection sensitivity; bioanalysis

"Estimation of parent flavonoids from supramolecular lipid complex of Punica granatum extract in rat serum by LC-ESI-MS/MS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 2, page no.f322-f326, February-2025, Available :http://www.jetir.org/papers/JETIR2502545.pdf

"Estimation of parent flavonoids from supramolecular lipid complex of Punica granatum extract in rat serum by LC-ESI-MS/MS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 2, page no. ppf322-f326, February-2025, Available at : http://www.jetir.org/papers/JETIR2502545.pdf