• Pooja Shah
• Dr. Joyline Mascarenhas

The increasing interest in green synthesis of products has led to an increased demand for microbial enzymes in industrial processes. In the present study, a protease-producing strain was isolated from a contaminated soil sample. The protease producers were screened on skim milk agar plates and the protease activity was confirmed using the Folin-Lowry method. Among the four isolates obtained in our study, Bacillus aryabhattai KJAM03 (NCBI accession no. LC337962) showed maximum protease production (32.12 U/mL). The ‘one factor at a time’ approach was followed to optimize the enzyme production from B. aryabhattai KJAM03 and a 73.17% improvement was achieved. The optimum conditions for enzyme production were identified as 1mL inoculum, 30°C, shaker conditions (130rpm) and 72h incubation in G medium (pH 7) containing peptone (5% w/v), casein (5% w/v) and 0.51% NaCl. The absence of peptone or casein reduced the protease production by 60.4% and 22.4% respectively. The enzyme activity was found to be stable between pH 6-10 and temperature up to 75°C. Although the loss of enzyme activity occurred in the presence of metal ions and surfactants, the enzyme showed excellent stability and compatibility with common laundry detergents (Ariel, Surf Excel and Tide). It also removed blood stains efficiently from cotton fabrics. Overall, our findings suggest a practical application of protease produced by B. aryabhattai KJAM03 in detergent formulations.

Bacillus aryabhattai, Detergent, Optimization, Protease, Surfactant

"The production, optimization and partial purification of protease enzyme from Bacillus aryabhattai KJAM03", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 3, page no.a763-a773, March-2025, Available :http://www.jetir.org/papers/JETIR2503095.pdf

"The production, optimization and partial purification of protease enzyme from Bacillus aryabhattai KJAM03", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 3, page no. ppa763-a773, March-2025, Available at : http://www.jetir.org/papers/JETIR2503095.pdf