• Priyanka payal

X-ray microscopy methods offer a unique application to biology with the ability to produce high-resolution three-dimensional images without sectioning. In recent years, lensless imaging techniques, such as coherent diffraction imaging (CDI) and ptychography, have been used to study biological systems by producing images with resolutions in the 30–50 nm range [1–3]. Sample preparation plays a key role in obtaining the highest resolution possible with these techniques while staying true to the native or natural state. Before doing synchrotron-based X-ray experiments, it is necessary to use other forms of microscopy for testing and optimizing sample preparation. Chromosomes are dynamic biological objects that undergo large morphological changes during the cell cycle; therefore it is difficult to establish their native state. The process of condensation sees the interphase chromatin become denser as the chromosomes start to form. During metaphase, chromosomes enter several stages of condensation before finally dividing.

Studies of Chromosomes with the Help of Scanning Electron Microscope

"Studies of Chromosomes with the Help of Scanning Electron Microscope", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 3, page no.b333-b343, March-2025, Available :http://www.jetir.org/papers/JETIR2503137.pdf

"Studies of Chromosomes with the Help of Scanning Electron Microscope", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 3, page no. ppb333-b343, March-2025, Available at : http://www.jetir.org/papers/JETIR2503137.pdf