• M. Prathibha Bharathi
• D.Vijaya Durga

TLC has recently gained popularity as an analytical method it may be used in a variety of contexts, particularly for the purpose of determining the presence of contaminants in a chemical. Prior to high-performance liquid chromatography (HPLC), it may be employed as a preliminary analytical procedure. TLC is easy to understand conceptually, and it requires little preparation of materials in most cases. TLC is the gold standard in many fields, including industrial chemistry, environmental toxicity, food chemistry, water, inorganic and pesticide analysis, dye purity, cosmetics, plant materials, and herbal analysis.In this study, an attempt was made to review the basic principles and the importance of Thin Layer Chromatography (TLC) in research in general. Thin layer chromatography is a simple, cost-effective, and easy-to-operate planar chromatographic technique which has been used in general chemistry laboratories for several decades to routinely separate chemical and biochemical compounds. Traditionally, chemical and optical methods are employed to visualize the analyte spots on the TLC plate. Also it has a wide application in identifying impurities in a compound. Study highlights the review on TLC and its application of qualitative and quantitative estimation of bio-active compounds from medicinal plants.

Keywords: Thin Layer Chromatography, Mobile phase , Stationary phase, UV cabinet.

"QUALITATIVE ANALYSIS OF ANTIDIABETIC DRUGS BY THIN LAYER CHROMATOGRAPHY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 6, page no.a802-a807, June-2025, Available :http://www.jetir.org/papers/JETIR2506083.pdf

"QUALITATIVE ANALYSIS OF ANTIDIABETIC DRUGS BY THIN LAYER CHROMATOGRAPHY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 6, page no. ppa802-a807, June-2025, Available at : http://www.jetir.org/papers/JETIR2506083.pdf