• Pushpesh Kumar
• Jyoti Verma
• Prof.(Dr.)Sanjay Kumar kushwaha3

A simple stability indicating high performance liquid chromatographic method has been developed for the simultaneous determination of Amlodipine in combination with metoprolol succinate using reverse phase Hypersil C18 column (150x4.6, 5μ) with UV detection at 230 nm. The mobile phase consisting of potassium dihydrogen phosphate buffer and acetonitrile in a ratio of 65:35 (v/v) PH was adjusted to 6.8±0.1 and at a flow rate of 1.0 mLmin-1.The method was linear over the concentration range for amlodipine 2.5-15 μgml-1 and for metoprolol 12-75 μgml-1. The recoveries of active pharmaceutical ingredient (API) Amlodipine and metoprolol succinate were found to be in the range of 100.10 – 101.16% and 98.50-100.02% respectively. The method was validated and was successfully employed for the routine quantitative analysis of pharmaceutical formulations containing Amlodipine and metoprolol succinate in combined tablet dosage form.

Amlodipine, Metoprolol, RP-HPLC, Validation and pharmaceutical formulations.

"METHOD VALIDATION BY UV AND RP-HPLC FOR QUANTITATIVE ESTIMATION OF METOPROLOL AND AMILODIPIN PURE AND PHARMACEUTICAL DOSEAGE FORMS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 7, page no.a203-a209, July-2025, Available :http://www.jetir.org/papers/JETIR2507019.pdf

"METHOD VALIDATION BY UV AND RP-HPLC FOR QUANTITATIVE ESTIMATION OF METOPROLOL AND AMILODIPIN PURE AND PHARMACEUTICAL DOSEAGE FORMS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 7, page no. ppa203-a209, July-2025, Available at : http://www.jetir.org/papers/JETIR2507019.pdf