• Dr.SUDHA PARIMALA
• AYESHA BEGUM
• BHUKYA NAGARAJU

Medicinal plants have been used for centuries in traditional systems of medicine for the treatment of various diseases. However, scientific validation and phytochemical characterization are essential to ensure their efficacy and safety. The present study aimed to investigate the phytochemical profile of Cissus pallida leaves, with a particular focus on tannin content. Specimens were collected from the Nallamala forest region (Kurnool, Nellore), authenticated by a taxonomist, and subjected to successive solvent extraction using petroleum ether, chloroform, and methanol. Preliminary phytochemical screening revealed the presence of steroids, alkaloids, flavonoids, tannins, and phenolics in varying amounts. Thin-layer chromatography (TLC) confirmed the presence of tannins exclusively in the methanolic extract. Quantitative estimation indicated that the leaves contain 3.14% w/w total tannins. These results suggest that Cissus pallida leaves may be a valuable source of tannins, which are known for their antioxidant, antimicrobial, and anti-inflammatory properties.

TLC,steroids, alkaloids, flavonoids, tannins, and phenolics.

"Phytochemical Investigation of Tannins in Cissus pallida Leaves", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 8, page no.c259-c263, August-2025, Available :http://www.jetir.org/papers/JETIR2508236.pdf

"Phytochemical Investigation of Tannins in Cissus pallida Leaves", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 8, page no. ppc259-c263, August-2025, Available at : http://www.jetir.org/papers/JETIR2508236.pdf