• Dr M Flory Shobana

The role of infectious pathogens bacteria in terms of severe human illnesses or even great deaths is undeniable. Over the last few years, the modern lifestyle has raised the problem of infectious pathogen bacteria more and more. Indeed, the rapid, sensitive and selective sensing platforms can prevent the spread of these microorganisms, additionally, optimize medical healthcare systems. In favor of this matter, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system, as a microbial defense system, has attracted considerable attention. To elaborate, although the system protects bacteria from being attacked by invading types, there are various types of developed biosensors using the CRISPR/Cas system for the determination of numerous targets, including bacteria. The shortcomings of current methods create an impending need for developing novel biosensing platforms. CRISPR-Cas systems, especially CRISPR-Cas12a and CRISPR-Cas13a, characterized by their sensitivity, specificity, high base resolution and programmability upon nucleic acid recognition, have been repurposed for molecular diagnostics, surging a new path forward in biosensing. They, as the core of some robust diagnostic tools, are revolutionizing the way that virus can be detected. This review focuses on recent advances in virus detection with CRISPR-Cas systems especially CRISPR-Cas12a/Cas13a.

CRISPR-Cas, pathogen detection, SHERLOCK, DETECTR, point-of-care diagnostics, nucleic acid biosensors, molecular diagnostics, infectious diseases

"CRISPR-CAS-BASED TECHNIQUES FOR PATHOGEN DETECTION: ADVANCES, APPLICATIONS, AND FUTURE PERSPECTIVES – A REVIEW", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 9, page no.d286-d300, September-2025, Available :http://www.jetir.org/papers/JETIR2509342.pdf

"CRISPR-CAS-BASED TECHNIQUES FOR PATHOGEN DETECTION: ADVANCES, APPLICATIONS, AND FUTURE PERSPECTIVES – A REVIEW", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 9, page no. ppd286-d300, September-2025, Available at : http://www.jetir.org/papers/JETIR2509342.pdf