• DR. V.SIVAJYOTHI
• SINLY FRANCIS
• SILPA C RAVI

The study aimed to evaluate the antifungal activity of a cream formulated using the ethyl acetate leaf extract of Artocarpus heterophyllus. The antifungal properties of the extract were assessed using the agar well diffusion method on a medium composed of Mueller-Hinton agar and Potato Dextrose Agar in a 1:1 ratio. This mixture was poured into sterile glass Petri plates and allowed to solidify. A standardized inoculum of Candida albicans was uniformly spread across the surface using a sterile cotton swab. Four wells, each 8 mm in diameter and spaced 20 mm apart, were aseptically punched into the agar using a sterile cork borer. The test samples (50 µL and 100 µL) were introduced into the wells. Clotrimazole (10 mg/mL) in two concentrations served as the positive control, while DMSO was used as the negative control. The cream’s physicochemical properties were found to be within acceptable limits. The minimum inhibitory zone diameters observed were 9 mm for 500 µg and 10 mm for 1000 µg of the extract and cream, respectively. A significant enhancement in antifungal activity was noted upon the addition of 2 g of pure A. heterophyllus extract to the cream formulation. The findings conclude that the ethyl acetate extract of A. heterophyllus and its herbal cream formulation exhibit promising antifungal activity against Candida albicans. This effect is likely due to the presence of bioactive phytoconstituents such as phenols, flavonoids, tannins, and alkaloids. The study suggests that this formulation holds potential for treating vaginal infections.

: Artocarpus heterophyllus; Candida albicans; Antifungal; Agar well diffusion

"EXTRACTION,FORMULATION AND EVALUATION OF HERBAL CREAM CONTAINING ETHYL ACETATE LEAF EXTRACT OF ATROCARPUS HETEROPHYLLUS FOR THE TREATMENT OF VAGINAL INFECTION.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 10, page no.a8-a24, October-2025, Available :http://www.jetir.org/papers/JETIR2510002.pdf

"EXTRACTION,FORMULATION AND EVALUATION OF HERBAL CREAM CONTAINING ETHYL ACETATE LEAF EXTRACT OF ATROCARPUS HETEROPHYLLUS FOR THE TREATMENT OF VAGINAL INFECTION.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 10, page no. ppa8-a24, October-2025, Available at : http://www.jetir.org/papers/JETIR2510002.pdf