• Ajay kumar
• dr. Vikash
• Sh. Deepak Kumar

This study focused on the investigation of antimicrobial effectiveness of green-synthesized silver nanoparticles (AgNPs), created using an extract from Citrullus colocynthis (Bitter Apple) leaves. The prepared AgNPs were tested for their antibacterial properties against the pathogens namely against gram positive- Bacillus cereus and gram negative Pseudomonas aeruginosa pathogenic bacteria. Driven by their ready availability and the avoidance of toxic chemicals, the demand for green synthesis of metallic nanoparticles has grown in various sectors, including electronics, water purification, catalysis, chemistry, and pharmaceuticals. The synthesized silver nanoparticles were characterized using UV spectroscopy, FT-IR, Zeta potential, and TEM. A color change from clear to colored upon mixing the plant leaf extract with an aqueous silver nitrate solution indicated the extract's ability to act as a reducing, capping, and stabilizing agent. Transmission Electron Microscopy (TEM) confirmed the nanoparticles' spherical shape, with a size range of 10–35 nm, and revealed their primary morphology and size distribution. FT-IR spectroscopy results, observed between 1000 and 4000 cm⁻¹, suggested the surface of the AgNPs was functionalized with free and bound amide groups, as well as polyphenols featuring an aromatic ring.

Citrullus colocynthis, Antimicroial property, Silver Nanoparticles

"Evaluation of Antimicrobial efficacy of Silver Nanoparticles synthesized using Leaf Extract of Citrullus Colocynthis ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 11, page no.a792-a801, November-2025, Available :http://www.jetir.org/papers/JETIR2511092.pdf

"Evaluation of Antimicrobial efficacy of Silver Nanoparticles synthesized using Leaf Extract of Citrullus Colocynthis ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 11, page no. ppa792-a801, November-2025, Available at : http://www.jetir.org/papers/JETIR2511092.pdf