• Amit Kumar Tamrakar
• Dr.Vikash Singh Thakur
• Kapil Kumar Chourasiya

The present investigation was undertaken to assess molecular polymorphism and genetic diversity among 11 pigeonpea (Cajanus cajan) genotypes and hybrids using Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR) markers. A total of 13 RAPD primers generated 97 scorable bands, of which 56 were polymorphic, resulting in an average polymorphism of 58.31%. The number of bands per primer ranged from 3 to 11, with OPA-16 producing the maximum polymorphic bands (7) and OPQ-12 the minimum (2). The polymorphism percentage varied from 28.57% (OPQ-12) to 83.33% (OPM-14), and the Polymorphic Information Content (PIC) ranged from 0.650 (OPG-12) to 0.897 (OPQ-14). Cluster analysis based on Jaccard’s similarity coefficient (ranging from 0.454 to 0.948) grouped the genotypes into two major clusters, revealing the highest genetic similarity between GT-101 and GT-102, and the lowest between GT-100 and CMS GT 288 A. RAPD markers effectively distinguished between parental lines and hybrids, with seven unique bands detected across genotypes. These findings underline the utility of RAPD markers for fingerprinting, diversity analysis, and parental line verification in pigeonpea breeding programs. While SSR markers also showed genetic variability, the RAPD technique proved more informative in this study, providing a robust and cost-effective tool for genetic analysis and varietal identification.

Keywords: Pigeonpea, Genetic Diversity, RAPD Markers, Polymorphism, Molecular Characterization

"Molecular Polymorphism in Pigeonpea Varieties and Hybrids Using RAPD and SSR Markers", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 11, page no.a813-a823, November-2025, Available :http://www.jetir.org/papers/JETIR2511094.pdf

"Molecular Polymorphism in Pigeonpea Varieties and Hybrids Using RAPD and SSR Markers", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 11, page no. ppa813-a823, November-2025, Available at : http://www.jetir.org/papers/JETIR2511094.pdf