• Mst. Sume Aktar Subarna
• Mst. Rokeya Sultana
• S.M Kamruzzaman
• Md. Hemayatul Islam
• Md. Wasiqur Rahman

Abstract Context: Antibiotic residues in broiler chickens pose a significant public health risk, contributing to antimicrobial resistance and potential direct toxicity to consumers. Background: The extensive use of antibiotics in poultry farming for therapy, prophylaxis, and growth promotion leads to residue accumulation in edible tissues. Understanding the distribution of these residues across different organs is crucial for risk assessment and food safety regulation. Methods: A cross-sectional study was conducted from January to March 2025 in Rajshahi City, Bangladesh. A total of 120 tissue samples (40 breast muscle, 40 liver, 40 kidney) were collected from broilers. Samples were analyzed for amoxicillin, tylosin, and ciprofloxacin residues using Thin Layer Chromatography (TLC). Data were analyzed using Chi-square tests. Results: The overall prevalence of antibiotic residues was 59.2% (71/120 samples tested positive for at least one antibiotic). Amoxicillin was the most prevalent (29.2%), followed by ciprofloxacin (24.2%) and tylosin (5.8%). Residue distribution was organ-dependent (p=0.0016). Amoxicillin residues were significantly higher (p=0.026) in the liver (48.6%) than in the kidney (34.3%) and breast muscle (17.1%). Ciprofloxacin showed a higher prevalence in the kidney (44.8%) compared to the liver (34.5%) and muscle (20.7%), though this was not statistically significant (p=0.145). Tylosin was predominantly found in the liver (57.1%), but the low number of positives rendered the tissue distribution non-significant (p=0.349). Conclusions: The high prevalence of antibiotic residues, particularly amoxicillin and ciprofloxacin, indicates non-prudent use of these drugs in broiler production. The liver and kidneys were identified as the primary reservoirs for residue accumulation. This practice threatens public health by fostering antimicrobial resistance and exposing consumers to drug residues. Strict adherence to withdrawal periods and regular monitoring of poultry products are essential to ensure food safety. Keywords: Broiler chicken, antibiotic residue, amoxicillin, ciprofloxacin, tylosin, TLC, organ prevalence.

"Comparative Analysis of Antibiotic Residue on Different Organ of Broiler Chicken", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 11, page no.b342-b348, November-2025, Available :http://www.jetir.org/papers/JETIR2511142.pdf

"Comparative Analysis of Antibiotic Residue on Different Organ of Broiler Chicken", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 11, page no. ppb342-b348, November-2025, Available at : http://www.jetir.org/papers/JETIR2511142.pdf