Abstract
ABSTRACT
In this study, a comparative analysis to identify the phytochemicals was done using different plant parts of Gmelina arborea Roxb., viz., inflorescence, leaves, and roots, for which different solvents like methanol, ethanol, acetone, and chloroform were employed to evaluate secondary metabolites like alkaloids, saponins, tannins, phenols, glycosides, proteins and amino acids, sterols, terpenoids, flavonoids, and carbohydrates. On extraction, the yield was observed between 3.25% to 12.8% based on the plant part and type of solvent, where ethanol was found to have the highest yield. Except for sterols and terpenoids, other secondary metabolites were tested positive in inflorescence extracts. Methanolic and ethanolic extracts of leaf contained all metabolites. On the basis of antioxidant studies using DPPH and ABTS assays, it was noted that ethanolic leaf extracts had the strongest radical scavenging activities (DPPH IC₅₀ = 22.4 ± 1.2 µg/mL; ABTS IC₅₀ = 28.7 ± 1.5 µg/mL, n = 3). Total phenolic content (TPC) and total flavonoid content (TFC) were found to be highest also in ethanolic leaf extracts (TPC = 138.5 ± 4.2 mg GAE/g extract; TFC = 102.3 ± 3.7 mg QE/g extract). From these findings, the remarkable medicinal importance of Gmelina arborea Roxb. is noted along with phytochemical screening, antioxidant potential.