Abstract
There had been insufficient research on the antioxidant properties of the three varieties of I. batatas, including Okinawa, NSIC 32, and Japanese tuberous roots. The objective of this study was to determine the antioxidant activities of three varieties of I. batatas tuber ethanolic extract. An experimental, post-test, completely randomized design, utilizing the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay in 3 trials and 5 replicates, was utilized. Using One-way ANOVA (p < .05) and Tukey’s hoc test, results showed that all concentrations, 0.25, 0.50, 0.75 mg/mL of I. batatas varieties Okinawa, NSIC 32, and Japanese exhibited antioxidant activity using the DPPH assay. The Okinawa at 0.25mg/mL (mean = 81.56, sd = .17), 0.50mg/mL (mean = 81.30, sd =.44), 0.75mg/mL (mean = 81.57, sd = .16), and 1.0mg/mL (mean = 84.44, sd = .11) exhibited antioxidant activity when compared to the negative control (mean = 0.00, sd = 0.00). The 100mg/mL Okinawa had comparable antioxidant activity to the positive control (mean = 85.1, sd = 0.00). The 0.25mg/mL, 0.50mg/mL, and 0.75mg/mL had comparable antioxidant activity. The best concentration for Okinawa was 1.0mg/mL. The NSIC 32 at 0.25mg/mL (mean = 80.32, sd = 1.12), 0.50mg/mL (mean = 81.16, sd = .44), 0.75mg/mL (mean = 81.71, sd = .11), 1.0mg/mL (mean = 82.03, sd = .32) had antioxidant activity too. All concentrations had comparable antioxidant activity, but not comparable to the positive control. The best concentration was 0.25mg/mL. The Japanese at 0.25mg/mL (mean = 77.23, sd = .25), 0.50mg/mL (mean = 79.1, sd = .18), 0.75mg/mL (mean = 79.90, sd = .17), and the 1.0m/mL (mean = 80.35, sd =.12) showed also comparable antioxidant activity but not comparable to the positive control. The best concentration was 0.25mg/mL. This research provided insights that Okinawa, NSIC 32, and Japanese I. batatas tuber as natural antioxidant agents.