• Blessie Grace Lopez
• Andrei Castellano
• ieka Mae Dionela, Arielle Angela Gallego, Mara Theresa Gallego, Ysabella Juliet Geolingo
• John Oliver Geresola, Erica Jade Gicaro, Precious Charlyn Gloria, Jann Annyle Gumalas, Leana Mae Go
• James L. Torres, Romagne John Palmos, Ferly Penaverde, Karen Ebreo, and Ma. Socorro Leong-on

The M. arvensis Linn. is known to have beneficial pharmacological and antioxidant properties, but its anti-angiogenic activity remains unexplored. The study aimed to determine the anti-angiogenic activity of the ethanolic extract of M. arvensis Linn. leaves using Anas platyrhynchos domesticus L (duck) chorioallantoic membrane (CAM) assay. Fertilized A. platyrhynchos domesticus L. eggs were treated with different concentrations (1 mg/mL, 0.5 mg/mL, 0.1 mg/mL, and 0.01 mg/mL) of the M. arvensis ethanolic extract, and blood vessel inhibition was assessed. The extract’s effects were compared to negative - Normal Saline Solution (NSS) and positive (Methotrexate) controls. The results exhibited an anti-angiogenic activity based on the number of secondary blood vessels (p≤0.05), 1 mg/mL (mean = 32, sd = 31), 0.5 mg/mL (mean = 41, sd = 31), 0.1 mg/mL (mean = 39, sd = 38), and 0.01 mg/mL (mean = 50, sd = 32). This was compared to the negative control (450 mg/500 mL NSS) (mean = 303, sd = 125). The anti-angiogenic activity of the different concentrations was comparable to one another and to the positive control (Methotrexate) (mean = 73, sd = 68). The best anti-angiogenic result was seen at 1 mg/ml of the crude ethanolic extract. This study supports the potential development of cost-effective, plant-derived anti-angiogenic agents that may benefit fields such as oncology and pharmaceutical development, subject for further study.

Mentha arvensis Linn., anti-angiogenic activity, CAM assay, ethanolic extract

"In Vitro Anti-angiogenic Activity of the Ethanolic Extract of Mentha arvensis Linn. (Yerba Buena) Leaves Using Duck Chorioallantoic Membrane (CAM) Assay", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.13, Issue 1, page no.d430-d434, January-2026, Available :http://www.jetir.org/papers/JETIR2601351.pdf

"In Vitro Anti-angiogenic Activity of the Ethanolic Extract of Mentha arvensis Linn. (Yerba Buena) Leaves Using Duck Chorioallantoic Membrane (CAM) Assay", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.13, Issue 1, page no. ppd430-d434, January-2026, Available at : http://www.jetir.org/papers/JETIR2601351.pdf