• Gore S F
• Dr D U Gawai

Abstract The present investigation was undertaken to isolate, identify, and assess the frequency of proteolytic fungi associated with stored seeds of Vigna radiata (Green gram), Cajanus cajan (Pigeon pea), Vigna mungo (Black gram), and Cicer arietinum (Chickpea). Stored pulses are highly susceptible to fungal contamination, leading to qualitative and quantitative losses. Many storage fungi secrete proteolytic enzymes that degrade seed proteins, thereby reducing nutritional value and germination capacity. Seed samples were collected from local storage facilities and markets. Fungi were isolated using the standard blotter and agar plate techniques on Potato Dextrose Agar (PDA). Identification was carried out based on macroscopic and microscopic characteristics using standard mycological keys. Proteolytic activity was screened using skim milk agar medium. A total of sixteen fungal species were recorded. Among these Aspergillus flavus, A. niger, and Penicillium citrinum were the most dominant species across all pulse seeds. The results indicate a high prevalence of proteolytic fungi in stored pulses, emphasizing the need for improved storage practices to minimize post-harvest losses.

Stored pulses, Proteolytic fungi, Seed mycoflora, Aspergillus, Protein degradation

"IDENTIFICATION AND ISOLATION OF SOME PROTEOLYTIC FUNGI FROM STORED PULSES SEEDS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.13, Issue 1, page no.d450-d455, January-2026, Available :http://www.jetir.org/papers/JETIR2601354.pdf

"IDENTIFICATION AND ISOLATION OF SOME PROTEOLYTIC FUNGI FROM STORED PULSES SEEDS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.13, Issue 1, page no. ppd450-d455, January-2026, Available at : http://www.jetir.org/papers/JETIR2601354.pdf