• Simran Raman Kumar Thakur
• Dnyanada Sadanand Ghadi
• Dr. Priyanka Pushkaraj Vartak
• Shweta Vijay Khopde

The antibacterial activity of Phyllanthus emblica (Amla) extracts prepared in different solvents; Dimethyl sulfoxide (DMSO), ethanol, and concentrated ethanol against two bacterial cultures: Escherichia coli and Staphylococcus aureus, is investigated in this study. Agar disk diffusion method was used to measure antimicrobial activity. The findings showed that the selection of the solvent and the recency of the extract had a direct impact on the antimicrobial activity of amla. For E. coli, the DMSO prepared extract contained the largest inhibition zone, reflecting higher bioavailability and solubility of active phytochemicals using this solvent. The concentrated ethanol extract contained the highest activity towards S. aureus, reflecting a more efficient extraction of effective bioactive compounds against Gram-positive bacteria. Interestingly, three-day stored amla extracts did not show antibacterial action against either of the microorganisms, highlighting the significance of extract freshness in maintaining bio-efficacy. The results highlight the importance of selecting the solvent and the stability of the extract in determining the most effective antimicrobial potential of the plant-based treatments. Such findings add to our improved knowledge of natural antimicrobial compounds and reveal the pharmaceutical value of amla in the form of natural herbal formulations. Future studies should aim to determine the precise bioactive constituents behind the antibacterial effect, evaluate their stability, and determine if there are synergistic interactions with traditional antibiotics. Such research will provide the foundation for new, environmentally friendly options to replace chemical antimicrobials.

Phyllanthus emblica, inhibition zone, Escherichia coli, Staphylococcus aureus, DMSO

"Assessment of Bioactive Efficacy of Phyllanthus emblica Extracts Against Bacterial Strains", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.13, Issue 3, page no.b703-b706, March-2026, Available :http://www.jetir.org/papers/JETIR2603185.pdf

"Assessment of Bioactive Efficacy of Phyllanthus emblica Extracts Against Bacterial Strains", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.13, Issue 3, page no. ppb703-b706, March-2026, Available at : http://www.jetir.org/papers/JETIR2603185.pdf