• P.SUBHA
• SYED BASUDEEN. B
• THAHIRAH BANU.M
• THRISHA.M
• UDAIYAMMAI.I, Dr. A. BABU THANDAPANI, Dr.S.RAMKUMAR

In this study, we chose traditionally used medicinal plants D. metel to examine their bioactive constituents and xanthine oxidase inhibition potential. Phytochemical screening of studied plant leaf extracts showed these plants as rich resources of secondary metabolites such as alkaloids, flavonoids, phenols, etc. Flavonoid are group of poly-phenolic compounds which have been proved to possess XO inhibitory activity. The XO inhibition assay was evaluated by UV-VIS spectrophotometry under aerobic conditions. The result of inhibition studies carried out by using Xanthine oxidase inhibitory assay clearly reveals that Datura metel at ethanol, ethyl acetate and aqueous tested extract at a concentration has inhibitory capacity compared to the standard drug (Allopurinol) at 50 to 200μg/ml. At a concentration 50μg/ml it shows a percentage inhibition 58.15% and 200μg/ml of plant it shows inhibition of 81.54% with an IC50 value 40μg/ml which is significantly similar to that of Allopurinol. Those data indicates that Ethanolic and ethyl acetate may have XO inhibitory activity.

Antioxidant, Xanthine oxidase inhibition, D. metel.

"PHYTOCHEMICAL ANALYSIS AND EVALUATION OF XANTHINE OXIDASE ACTIVITY OF DATURA METEL LEAVES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.13, Issue 3, page no.h259-h263, March-2026, Available :http://www.jetir.org/papers/JETIR2603733.pdf

"PHYTOCHEMICAL ANALYSIS AND EVALUATION OF XANTHINE OXIDASE ACTIVITY OF DATURA METEL LEAVES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.13, Issue 3, page no. pph259-h263, March-2026, Available at : http://www.jetir.org/papers/JETIR2603733.pdf