• AJRU DEEN
• Dr. Bhupendra Kumar Kumawat

Oxidative stress and pathological angiogenesis play critical roles in the progression of chronic inflammatory disorders and cancer. The present study evaluated the antioxidant and anti-angiogenic potential of hydroalcoholic (70% v/v ethanol) extracts prepared from the root and stem bark of *Calotropis procera* collected from the Sariska Tiger Reserve, Alwar, Rajasthan. Extracts were obtained by cold maceration, yielding 12.40% for root and 16.80% for bark. Antioxidant activity was assessed using the 2,6-dichlorophenolindophenol (DCPIP) decolorization assay with ascorbic acid as standard. Both extracts exhibited concentration-dependent activity; the root extract showed superior potency (IC₅₀ = 185.6 µg/mL) compared to the bark extract (IC₅₀ = 238.4 µg/mL), while ascorbic acid displayed an IC₅₀ of 14.2 µg/mL. Anti-angiogenic activity was evaluated using the HUVEC tube formation assay on Matrigel. Root and bark extracts inhibited VEGF-induced tube formation in a dose-dependent manner, with IC₅₀ values of 132.4 ± 8.7 µg/mL and 212.6 ± 11.3 µg/mL, respectively. Suramin (standard) showed an IC₅₀ of 28.6 ± 2.1 µg/mL. Microscopic observations confirmed progressive disruption of capillary-like networks with increasing extract concentrations. The findings demonstrate that hydroalcoholic root extract of *C. procera* possesses stronger dual antioxidant and anti-angiogenic activity than the bark extract, supporting its potential as a multi-target phytotherapeutic agent against oxidative stress-related and angiogenesis-driven disorders.

Calotropis procera; hydroalcoholic extract; antioxidant activity; DCPIP assay; anti-angiogenic activity; HUVEC tube formation; IC₅₀; Sariska Tiger Reserve.

"Evaluation of Antioxidant and Anti-Angiogenic Potential of Root and Stem Bark Extracts of Calotropis procera selected from Sariska Tiger reserve Alwar ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.13, Issue 9, page no.a555-a562, September-2026, Available :http://www.jetir.org/papers/JETIR2609067.pdf

"Evaluation of Antioxidant and Anti-Angiogenic Potential of Root and Stem Bark Extracts of Calotropis procera selected from Sariska Tiger reserve Alwar ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.13, Issue 9, page no. ppa555-a562, September-2026, Available at : http://www.jetir.org/papers/JETIR2609067.pdf