• Dr.S. Elamathy
• Dr.T. Prabu
• M. Kaviya
• D. Kayathri

Medicinal plants represent an important source of bioactive compounds with potential therapeutic applications. The present study aimed to formulate and evaluate a herbal ointment containing extracts of turmeric (Curcuma longa), eucalyptus (Eucalyptus globulus) leaves, and ginger (Zingiber officinale) and to assess its antimicrobial potential. The collected plant materials were washed, processed into a fine paste, and extracted with ethanol by maceration for 3–5 days. The resulting crude extract was filtered and subjected to qualitative phytochemical screening. The screening revealed the presence of several phytoconstituents, including alkaloids, carbohydrates, glycosides, saponins, proteins, amino acids, phenolic compounds, flavonoids, terpenoids, phlobatannins, lignins, resins, lactones, anthocyanins, and betacyanins. A semi-solid herbal ointment was prepared using white wax and yellow paraffin as the base, followed by incorporation of the plant extract. The formulated ointment was evaluated for physicochemical characteristics, including color, odor, consistency, pH, spreadability, and stability. Its antimicrobial activity was evaluated against three bacterial strains, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, and three fungal strains, Aspergillus niger, Candida albicans, and Penicillium sp., using the agar well diffusion method. The formulated ointment exhibited inhibitory activity against the tested bacterial and fungal strains, indicating its potential antimicrobial properties. The observed activity may be associated with the combined effects of the bioactive phytochemicals present in the selected plant extracts. Overall, the findings suggest that the formulated herbal ointment has potential as a plant-based topical formulation for further investigation against microbial skin infections. Further studies involving quantitative phytochemical analysis, optimization of formulation, toxicity assessment, and statistical evaluation are warranted to establish its efficacy and safety.

Curcuma longa; Eucalyptus globulus; Zingiber officinale; herbal ointment; phytochemical screening; antimicrobial activity; topical formulation.

"PREPARATION AND EVALUATION OF HERBAL OINTMENT FROM SELECTED MEDICINAL PLANTS AND ITS ANTIMICROBIAL ACTIVITY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.13, Issue 10, page no.b23-b38, October-2026, Available :http://www.jetir.org/papers/JETIR2610104.pdf

"PREPARATION AND EVALUATION OF HERBAL OINTMENT FROM SELECTED MEDICINAL PLANTS AND ITS ANTIMICROBIAL ACTIVITY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.13, Issue 10, page no. ppb23-b38, October-2026, Available at : http://www.jetir.org/papers/JETIR2610104.pdf