• Yaswobanta Kar
• Saswati Das
• Preetha Bhadra
• Bishwambhar Mishra

Review of literature has showed that there is a high demand for type “O” blood. Type-o positive is the most transfused blood type as it can be given to any patients with RH positive blood type due to absence of antigens and presence of both antibodies. Meanwhile type o-negative is a universal donor and can be given to patient of any blood type due to the absence of any kind of antigens. The eight different blood types that can be categorized into four groups based on their antigens: A, B, AB, and O with the help of presence on antigens and antibodies which are responsible for the detection of the foreign objects and deal with it. Our aim was to convert the AB blood group to O blood group. Thus it can be safely transfused to anyone without any risk. We have extracted the total DNA from human gut using metagenomics and expressed in E. coli. It eventually led to focus on the mucosal lining of the human gut, which contains sugars that similar in structure to blood antigens. They were then able to “use E. coli to select for DNA containing genes that code for enzymes that can cleave sugar residues.” then multiple the enzymes and found that it was capable of removes the Gal or Gal-NAc residues that determine the antigens of type-A and type-B blood, which essentially makes the blood type-O.2) select specific glycoside hydrolase families of interest and create a phylogenetically distinct sub-families of their genes. From each sub-family select a representative gene and expressed it in E.coli.

Blood Group, E.coli, Human Gut, Metagenomics, Phylogenetics

"A NOBLE AND INNOVATIVE SOLUTION TO BLOOD SHORTAGE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 9, page no.259-261, October-2018, Available :http://www.jetir.org/papers/JETIRA006364.pdf

"A NOBLE AND INNOVATIVE SOLUTION TO BLOOD SHORTAGE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 9, page no. pp259-261, October-2018, Available at : http://www.jetir.org/papers/JETIRA006364.pdf