• Bhavya G
• Abhijith P
• Ravikant S
• Geetha N

In nature, the microorganism’s, especially fungi are developed with a different metabolic strategy’ to combat the adverse effects of metal-induced toxicity thereby decreasing their scathe. In the current work, the Candida sp. strain Y-01 and SIE - 01 have shown resistance to Pb2+ in the concentration range of 0.2mM to 2.0mM. Further exploring the distinguishable cause of resistance, the antioxidant defence enzyme system and the membrane integrity of cells were analyzed with respect to 0.8mM concentration of Pb2+ at 72hr of incubation. On evaluating the CAT and MDA contents, the increased CAT and decreased MDA activities was observed in Candida sp. strain Y-01 in comparison with the control. The increased CAT indicates the possible key role of CAT related pathway for the resistance mechanism. The decreased MDA indicated the lower oxidative stress to membrane component, which was justified as membrane integrity of cell found to be around 85% upon stress with respect to the control. The increased CAT, MDA and decreased membrane integrity (45%) were observed in SIE – 01. The increased MDA indicated the oxidative lipid peroxidation of cell membrane component thus leading to loss of cell membrane integrity, changes in membrane fluidity and metabolic process in the yeast isolate SIE -01. The results suggest that the application of Candida sp. strain Y-01 for bioremediation of Pb2+ contaminated site and use of CAT and MDA as biomarkers for metal-induced stress.

– Candida sp., CAT, MDA, Pb2+

"A STUDY ON ANTIOXIDANT ACTIVITY AND MEMBRANE INTEGRITY OF CANDIDA SP. STRAIN Y-01 UPON PB STRESS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.8-12, March-2019, Available :http://www.jetir.org/papers/JETIRAD06002.pdf

"A STUDY ON ANTIOXIDANT ACTIVITY AND MEMBRANE INTEGRITY OF CANDIDA SP. STRAIN Y-01 UPON PB STRESS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp8-12, March-2019, Available at : http://www.jetir.org/papers/JETIRAD06002.pdf