• Dr. K.Kalaiarasi

An efficient invitro propagation method was optimized using nodal explants from Pogostemon cablin by organogenesis. Regeneration protocol standardized on MS medium supplemented with BAP (1.5 mg/l). The maximum number of callus was developed from the leaf and node explants in the presence of 2,4-D (1.0 mg/l) and BAP (0.5 mg/l). The maximum number of shoot regeneration (5.5) was observed in MS medium with BAP (2.0 mg/l), KIN (1.5 mg/l) and NAA (1.5 mg/l). The maximum frequency (85.3) and number of rootlets (7.5) were obtained in MS medium with IAA (1.5 mg/l). After one month of rooting, the plantlets were transferred to the polycups containing mixture of sterile garden soil and sand (3:1). The plants were acclimatized successfully with 90% in pots under green house condition. The plants were also successfully established in field condition with 85% of survival rate.

Pogostemon cablin; Micropropagation; Nodal explants

"Optimization of invitro propagation for Pogostemon cablin from Western Ghats of Tamil nadu", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.165-175, March-2019, Available :http://www.jetir.org/papers/JETIRAD06026.pdf

"Optimization of invitro propagation for Pogostemon cablin from Western Ghats of Tamil nadu", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp165-175, March-2019, Available at : http://www.jetir.org/papers/JETIRAD06026.pdf