• Nilesh Sonune
• Shubham Rakh

In recent years, microbial L-asparaginases have drawn particular attention because of their potential antineoplastic properties and significant application in food industries. In present study, total 67 bacterial isolates were isolated and screened for L-asparginase production from agricultural soil on a Modified M9 agar medium with phenol red as an indicator. Among 67, 19 isolates showed L-asparginase production ability. Among which one isolates Bacillus sp. showed highest production and was optimized for various condition for better results. The result suggests that optimum pH, temperature, source of carbon and source of nitrogen for L- asparginase production by Bacillus sp. was 8, 370C, 0.8% glucose and 1.5% yeast extract, respectively.

Bacillus sp., enzymes, L- asparginase, optimization

"Isolation, screening and optimization of L-asparginase producer Bacillus sp. from agricultural soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.68-72, May-2019, Available :http://www.jetir.org/papers/JETIRBL06012.pdf

"Isolation, screening and optimization of L-asparginase producer Bacillus sp. from agricultural soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp68-72, May-2019, Available at : http://www.jetir.org/papers/JETIRBL06012.pdf