• Hiteshree Lokhande
• Sonali Nimbhorkar

Metagenomic analysis process include understanding of symbiotic systems and effects of environment pollutions. However, metagenome analysis requires sequence homology searches which require large computation time and it is thus a challenge in current metagenome analysis based on the data from the latest DNA sequencers generally called a next-generation sequencer. Metagenome analysis is the understanding of the genomes of microbes which is obtained directly from microbial communities in their natural habitats such as soils, seas, and human bodies. It includes genomic sequences which is obtained directly from environmental microbial communities with the motive of linking their structures with functional roles. The major problem of this work is to find distribution of phenol degradation of genes and tracing any possible variations in its genes across various sources. The increased antibiotic resistance among microorganisms has resulted into growing interest for investigating the wastewater treatment plants (WWTPs) as they are reported to be the major source in the dissemination of antibiotic resistance genes (ARGs) and heavy metal resistance genes (HMRGs) in the environment. Biological wastewater treatment is among the most important biotechnological applications and, as drivers of the key processes, microorganisms are central to its success. Therefore, the study of wastewater microorganisms has obvious applied significance. Metagenomes also referred to as environmental and community genomics is the genomic analysis of microorganisms by direct extraction and cloning of DNA from an assemblage of microorganisms.. Metagenome consist of genetic material of environmental samples, here the computational tools are used,using it we can study the protein sequences of different bacteria and compare their genetic information present in them and can then classify them into different bacteria according to genetic analysis.The challenges we faced is metagenomes are taken from different places,their protein structure is different from genomes,understanding their characteristics, collection of those sequences from database takes long time,so using some computational tools time taken to perform these task should be minimized.

Metagenomics,WWTPs,ARGs, HMRGs

"Analysis of Metagenomes of Wastewater", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.43-49, May-2019, Available :http://www.jetir.org/papers/JETIRBM06008.pdf

"Analysis of Metagenomes of Wastewater", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp43-49, May-2019, Available at : http://www.jetir.org/papers/JETIRBM06008.pdf