• KRUPALI N. DAMANIA
• URBI KUNDU

Real time PCR is currently considered as the gold standard method for detection of plant pathogen. Real time PCR aiming at quantifying the level of plant infection by viral pathogens are becoming more and important within microbiology. Real time PCR is frequently used in gene expression studies as it fits perfectly with it wide dynamic range, sensitivity, and ease of automation possibilities. This technique allows the monitoring of the reaction during the amplification process by using of a fluorescent signal that increases proportionally to number of amplicons generated and to the number of target present in the samples. Real time PCR is the reliable and provides high throughput quantification of target viral pathogens DNA in various environmental samples, including hosts tissues, soil, and air. Real time PCR has versatile practical application in diagnostic of plant disease. Monitoring of health and detection of diseases in plants is critical for sustainable agricultural. Till now 85% of viral diseases have been diagnosed in virus infected plants. Real time PCR plays a significant role in better understanding of the dynamic of plant pathogenic microbes and thereby allows better management of diseases.

Real time PCR, viral pathogens, Quantification, fluorescent, diagnosis

"Standard Quantification of gene expression in viral infected plant by using Real Time PCR ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.334-337, May-2019, Available :http://www.jetir.org/papers/JETIRBM06052.pdf

"Standard Quantification of gene expression in viral infected plant by using Real Time PCR ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp334-337, May-2019, Available at : http://www.jetir.org/papers/JETIRBM06052.pdf