• Talib Hussain
• Anjali Bhargava

Copper sulfate is a chemical which is used widely in the agricultural sector and in fishery sector but at high concentration it affect the aquatic life. To check the toxicity of copper on fishes this experiment is conducted. Two aquariums were used for the experiment, one is for control and the other is for experimental purpose but three aquariums with 10 fishes each were used to determine LC50 and was calculated by using probit analysis method which was 23.4ppm. Graph on dose response relationship was also constructed which was sigmoid shaped. Correlation between mortality percentage and dose was also calculated which showed perfect positive correlation. Hardness and alkalinity was also measured daily and average was 188.4058ppm and 135ppm respectively. Temperature was kept constant at 25 ⁰C. For the experiment two aquariums with 10 fishes each were setup. In the experimental group fishes were treated with 2.3ppm copper sulfate dose for 15 days. 30% water was changed after every 48 hours. After 15 days slides of gills and brain of both experimental and control group were prepared and comparative study was done to check the effects. The normal structure of gills were highly altered. Hyperplasia , fusion of two secondary lamella, change in axis of secondary lamella, swelling of cells in primary lamella and basement membrane, buldging of the tips of secondary lamella were reported. In brain optic lobes showed some adverse effect such as Vacuolization, Spongiosis, and proliferation of the optic tectum occurred. Cells of optic tectum proliferated into the granular layer of valvula cerebellii. No histopathological changes observed in cerebellum and medulla ablongata.

LC50,Hyperplasia, Spongiosis, Vacuolization, Proliferation, Optic tectum, Valvula cerebelli.

"Histopathological Changes in the Gills and Brain of Cyprinus carpio Exposed to copper sulfate", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.419-430, May-2019, Available :http://www.jetir.org/papers/JETIRCJ06095.pdf

"Histopathological Changes in the Gills and Brain of Cyprinus carpio Exposed to copper sulfate", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp419-430, May-2019, Available at : http://www.jetir.org/papers/JETIRCJ06095.pdf