• Hephzibah Rani
• B.V.Sandeep
• M.S.Chakravarthy

The present study reports purification of extracellular glutaminase enzyme from streptomyces parvus . Screening was performed from twenty Actinomycetesisolates from soil; one isolate (Isolate HSBT0318) was finally selected based on the activity of glutaminase(36.48 U/ml).The isolate was identified as Streptomyces sp. The L-glutaminase produced from Streptomyces parvus was purified by ammonium sulphate precipitation, dialysis method and ionexchange chromatography. After the purification of the enzyme by ion exchange chromatography, it has been purified 46-fold from cell-free extract and yield was 3.25%. Characterization of extracellular L-glutaminase showed optimal activity at temperature of 30°C, pH 7, at 3% NaCl and for 0.04M substrate and the Km value was calculated to be 2.8mM and Vmax was 7.57 U/ml. The molecular weight of enzyme as determined by sodium dodecyl sulphate polyacrylamide electrophoresis(SDS-PAGE) was found to be 45 kDa.

L-Glutaminase, Actinomycetes, Marine sediments, Streptomyces.sp, ion exchangechromatography, SDS-PAGE.

"Purification of anti cancerous enzyme L-glutaminase from marine Streptomyces parvus HSBT0318 ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.739-746, May-2019, Available :http://www.jetir.org/papers/JETIRCJ06155.pdf

"Purification of anti cancerous enzyme L-glutaminase from marine Streptomyces parvus HSBT0318 ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp739-746, May-2019, Available at : http://www.jetir.org/papers/JETIRCJ06155.pdf