UGC Approved Journal no 63975(19)

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Published in:

Volume 6 Issue 5
May-2019
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIRCU06130


Registration ID:
218357

Page Number

701-711

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Title

Isolation and Phylogenetic Identification of Seed borne Mycoflora associated with Rice and Eco-friendly management by Datura stramonium with Phytochemical Screening : GC-MS analysis

Abstract

The study illustrates the efficiency of Antifungal activity of ethyl acetate extract of Datura stramonium against some seed borne fungi (Aspergillus sp, Rhizopus sp, Fusarium sp, and Macrophomina sp.) isolated from field crop Rice (Variety- Khas) of Indo-Gangetic area, using standard microbiological procedures and to investigate the active compound of ethyl acetate extract of Datura stramonium which is responsible for antifungal activity by GC-MS technique. The identification of fungi was carried out by phylogenetic tree construction and 18S rRNA sequencing. The well diffusion method was used to study the efficacy of Datura stramonium . The extracts were poured into the wells at different concentrations including 50 mg/ml, 100 mg/ml, 200 mg/ml and 400 mg/ml. After incubation the zone of inhibition was carried out by agar plate method was observed. The concentration of 400 mg/ml showed the maximum zone of inhibition (18.8mm±0.2) where as the concentration of 50 mg/ml showed the minimum zone of inhibition (12.7mm±0.1) for isolated Fusarium oxysporum. Study has also been shown the presence of Bio active compound such as N-(2-Bromobutyryl)-4-chloro-benzenesulfonamide in the leaf of Datura stramonium which is responsible for antifungal activity and which can be used for seed treatment against seed borne fungal disease.

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"Isolation and Phylogenetic Identification of Seed borne Mycoflora associated with Rice and Eco-friendly management by Datura stramonium with Phytochemical Screening : GC-MS analysis", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.701-711, May 2019, Available :http://www.jetir.org/papers/JETIRCU06130.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Isolation and Phylogenetic Identification of Seed borne Mycoflora associated with Rice and Eco-friendly management by Datura stramonium with Phytochemical Screening : GC-MS analysis", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp701-711, May 2019, Available at : http://www.jetir.org/papers/JETIRCU06130.pdf

Publication Details

Published Paper ID: JETIRCU06130
Registration ID: 218357
Published In: Volume 6 | Issue 5 | Year May-2019
DOI (Digital Object Identifier):
Page No: 701-711
Country: Chennai, Tamil Nadu, India .
Area: Engineering
ISSN Number: 2349-5162
Publisher: IJ Publication


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