UGC Approved Journal no 63975(19)

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Published in:

Volume 5 Issue 8
August-2018
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIRD006017


Registration ID:
186564

Page Number

97-111

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Title

Stability-indicating RP-HPLC method for the determination of doxepin, benzyl alcohol and capsaicin in bulk & pharmaceutical dosage forms

Abstract

An accurate, rapid economical and simple, reliable method was developed and validated for the simultaneous estimation of Doxepin, Benzyl alcohol and capsaicin using RP-HPLC. In the proposed method efficient chromatographic separation was achieved using symmetry C18 column (150mmx4.6mm, dp=3.5µm) as a stationary phase and acetonitrile:buffer (40:60v/v, 3.0 gm octane-1-sulphonic acid in 1lt water adjust pH-2.5 with OPA) as a mobile phase with a flow rate of 1.0 ml/min and UV detection at 273nm.Chromatography was carried out isocratically at ambient temperature and the run time was 12min. In this method a good linearity was observed in the range of 6.6-99 µg/ml, 2-30 µg/ml and 0.1-1.5 µg/ml with the limit of detection(s/n=5,4and7) 0.06µg/ml, 0.02µg/ml and 0.001µg/ml for doxepin, benzyl alcohol and capsaicin respectively. The retention time for capsaicin, doxepin and benzyl alcohol were 3.57, 6.10 and 9.10 minutes respectively. The proposed method was validated as per ICH Q2(R1) guidelines.

Key Words

benzyl alcohol; capsaicin; high performance liquid chromatography.

Cite This Article

"Stability-indicating RP-HPLC method for the determination of doxepin, benzyl alcohol and capsaicin in bulk & pharmaceutical dosage forms", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 8, page no.97-111, August-2018, Available :http://www.jetir.org/papers/JETIRD006017.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Stability-indicating RP-HPLC method for the determination of doxepin, benzyl alcohol and capsaicin in bulk & pharmaceutical dosage forms", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 8, page no. pp97-111, August-2018, Available at : http://www.jetir.org/papers/JETIRD006017.pdf

Publication Details

Published Paper ID: JETIRD006017
Registration ID: 186564
Published In: Volume 5 | Issue 8 | Year August-2018
DOI (Digital Object Identifier):
Page No: 97-111
Country: --, -, - .
Area: Engineering
ISSN Number: 2349-5162
Publisher: IJ Publication


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