• Shashank Garg

Bacillus subtilis RKR-14 is utilized to produce protease. The activity of protease was measure at 37 C and 5 pH. Growth curve was plotted and it was seen that the organism achieved its log phase at about 6 hrs which was then utilized to harness active culture. The organism was found to be gram positive rod. This actively growing culture was optimized for temperature and pH conditions for production of protease and it was revealed that pH 5 and 50 C were the best among the tested states for highest enzyme activity. Further a two-way ANOVA technique was utilized to access the combinatorial effect of both the factors on enzyme activity. It was seen that while temperature has a profound effect on the enzyme activity, pH was found to affect it not so significantly. In all, the organism was found to be a potent candidate for the production of protease.

pH, ANOVA, Protease

"Production and Optimization Of Production Of Bacterial Protease", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.514-522, December 2018, Available :http://www.jetir.org/papers/JETIRDT06068.pdf

"Production and Optimization Of Production Of Bacterial Protease", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp514-522, December 2018, Available at : http://www.jetir.org/papers/JETIRDT06068.pdf