• Sanjeet Singh
• Nilesh Talekar
• Puneet Walia

EcoTILLING is a modification in the TILLING strategy where naturally occurring SNPs are” targeted in specific genes. This method examines a cultivar/ inbred line/accession against a” “reference” genome in a one-on-one comparison to a reference genome, usually the genome that has been sequenced (where that is an option) (Comai et al, 2003). SNPs are detected in the same” “manner that induced mutations are in EMS TILLING. There are often more than one such SNP”” “within a target in EcoTILLING, and there will therefore often be multiple bands in a lane.” Patterns unique to individual accessions, relatively rare SNPs common among only a small number of” “accessions, and more common SNPs are all visible on the same gel. Comparison of many different accessions to a reference genome by EcoTILLING can define unique patterns for individual” “accessions, identify patterns of SNPs shared among accessions that suggest relatedness, and reveal differing levels of selection in “different regions of genes (e.g. decreased diversity within exons versus introns) as well as among genes. Valuable, practical information can be gotten from EcoTILLING analysis as well. For example, analysis of simple sequence repeat markers from ESTs reveals very high levels of divergence between accessions. In domesticated crop species, EcoTILLING has also proven to be a useful, relative measure of the sequence diversity remaining in highly inbred, elite germplasm.

EcoTILLLING, Reverse genetics, Genome-Wide Mutagenesis, crop improvement.

"EcoTILLING: A POTENT REVERSE GENETICS TOOL", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 1, page no.871-875, January 2019, Available :http://www.jetir.org/papers/JETIRDW06138.pdf

"EcoTILLING: A POTENT REVERSE GENETICS TOOL", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 1, page no. pp871-875, January 2019, Available at : http://www.jetir.org/papers/JETIRDW06138.pdf