• Najuruddin
• Daljeet Singh Dhanjal
• Reena Singh Chopra
• Chirag Chopra

Amylases are highly prevalent in environment and perform an imperative role in the field of biotechnology, as they share around 25% of global enzyme market. This enzyme induces the hydrolysis of internal alpha 1,4-glycosidic bonds of starch to produce glucose. The wide range of the applications of amylases in the various processes of industries like fermentation, textile, food, paper, detergent and in pharmaceutical industries has impelled the exploration of the unique and extreme regions to isolate the amylase-producing bacteria. The current study focusses on isolating the extremophilic amylase-producing bacteria from the crop fields of Pathankot region. After isolation, the isolated amylase producing strain was optimized, for which optimal activity at 90oC and pH 5.0. Additionally, the metal ion like MnCl2 and CoCl2 were found to be positive co-factor for enhancing enzyme activity, whereas, ZnCl2 and CaCl2 showed the inhibiting effect on the enzyme. Further, the bacteria showing the optimum activity was found to be gram negative and rod-shaped (phenotype similar with Bacillus).

Amylase, Culture Dependent, Extremophile, Optimization, Pathankot

"Identification and Biochemical Characterization of a Thermophilic Amylase from the Microbes in the Soil of Pathankot Region", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.524-529, December 2018, Available :http://www.jetir.org/papers/JETIRDZ06068.pdf

"Identification and Biochemical Characterization of a Thermophilic Amylase from the Microbes in the Soil of Pathankot Region", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp524-529, December 2018, Available at : http://www.jetir.org/papers/JETIRDZ06068.pdf