UGC Approved Journal no 63975(19)

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 9 Issue 5
May-2022
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIRFO06054


Registration ID:
405455

Page Number

307-313

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Title

In vitro Micropropagation of Tinospora cordifolia - An Important Medicinal Plant

Abstract

A protocol was established for rapid clonal propagation medicinal plant, Tinospora cordifolia (TC), through in vitro culture using nodal explants. Best shoot induction was observed on MS medium with 4.36 µM KIN produces 2.320.1 cm length with 1.80.1 numbers of shoots with 70% response when compared with other Cytokinin’s BA and 2iP. After a month of shoot induced culture, creates a phenolic exudation problem in shoot formation, media had discoloration and explants were browning and the buds were break so, further analysis protocols were found by treating with different substances from that, silver nitrate (20%) with KIN (4.36 µM) gives 100% response with 3.011.0 cm length of shoots with 2.010.1 numbers of shoots within 16 days. Nodal explants of TC were cultured on MS medium with various concentrations of BA alone and in combination with NAA or IAA for shoot proliferation. From that, BA (8.82 µM) alone showed better growth response and produced 4.810.2 numbers of shoots with an average length of 3.10.1 cm after 20 days. Small sheetlet were transferred to shoot elongation medium with 8.82 µM of BA alone. An average length of 4.820.4 cm with 4.610.2 numbers of shoots produced, 76% of response. The elongated shoot lets transferred to half strength MS medium and 6.43 µM of IBA with 3% sucrose produce 5.20.2 rootlets per plant with average root length of 3.20.1 cm after 27 days. Rooted plantlets were transplanted ex vitro. Approximately 80% of plantlets survived.

Key Words

Micropropagation, Tinospora cordifolia, Nodal explants, Shoot proliferation

Cite This Article

"In vitro Micropropagation of Tinospora cordifolia - An Important Medicinal Plant ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 5, page no.307-313, May-2022, Available :http://www.jetir.org/papers/JETIRFO06054.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"In vitro Micropropagation of Tinospora cordifolia - An Important Medicinal Plant ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 5, page no. pp307-313, May-2022, Available at : http://www.jetir.org/papers/JETIRFO06054.pdf

Publication Details

Published Paper ID: JETIRFO06054
Registration ID: 405455
Published In: Volume 9 | Issue 5 | Year May-2022
DOI (Digital Object Identifier):
Page No: 307-313
Country: -, -, India .
Area: Engineering
ISSN Number: 2349-5162
Publisher: IJ Publication


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