• Jitendra Kumar Meshram
• Dr. Praveen Kumar

Superoxide dismutase is an enzyme that catalysis the reversible reduction of the damaging superoxide (O2) radical into the inert molecular oxygen (O2) and hydrogen peroxide (H2O2) (H 2O 2). Aerobic organisms produce superoxide, a kind of reactive oxygen species (ROS), as a byproduct of their respiratory metabolism. Through the Fenton reaction, hydrogen peroxide (H2O2) and ferric iron (Fe2+) may generate the reactive oxygen species (ROS) hydroxide ion (•HO). H2O2 is an essential sensor in redox metabolism. According to Hirs' performic acid oxidation technique, we measured total cysteine and half-cystine in triplicate. spectrophotometric approach was used to determine the concentration of tryptophan. Dimethyl sulfoxide was used in the Spencer and World technique for determining total cysteine. Differential centrifugation in isotonic sucrose is a well-established technique for isolating mitochondria from chicken liver. In order to prevent interference from cytochrome c peroxidases and oxidases, superoxide dismutase was measured in the same way as reported before (4), with the addition of 1 x 1O3 M cyanide.

antioxidant; superoxide dismutase; supplementation; detoxification

"INVESTIGATION OF THE COMPLEXES FOR SUPEROXIDE DISMUTASE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 10, page no.31-40, October-2022, Available :http://www.jetir.org/papers/JETIRFU06004.pdf

"INVESTIGATION OF THE COMPLEXES FOR SUPEROXIDE DISMUTASE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 10, page no. pp31-40, October-2022, Available at : http://www.jetir.org/papers/JETIRFU06004.pdf