• Jaiwal BhimraoVishwanath
• Yuvraj Prakash Kale
• Patil Ajit Babruwahan

Previous studies recommended that salivary and pulp-derived host matrix metalloproteinases (MMPs) participate in dentin caries pathogenesis. In this study we assessed in-vitro inhibitory effect and mechanism of action of standard phenolic compounds such as caffeic, p-hydroxybenzoic, coumaric, gallic and syringic acid against human salivary metalloproteinases.Gelatin zymography revealed six prominent gelatinalytic bands (P1, P2, P3, P4, P5, and P6) from human concentrated saliva out which P3, P4, P5 and P6 were confirmed as metalloproteinases. All standard phenolics exhibited dose-dependent inhibition against salivary metalloproteinases as well as metal chelating activity.Phenolic acids range 1-2 mM concentration required for complete inhibition of metalloproteinase on zymography. Caffeic acid exhibited maximum inhibition and chelating activity (93.75 IC50 µg/ml) as compared to other phenolics. Inhibitory effect of phenolic compound may be due to metal chelating action was concluded. Results of this study suggested that further study is needed to see the inhibitory effect of phenolic compounds against individual salivary MMP.

Gelatin zymography, Standard phenolics, Caffeic acid, Chelating activity.

"In-Vitro Inhibitory Effect of Standard Phenolic Compounds against Human Salivary Metalloproteinases", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 4, page no.385-393, April-2023, Available :http://www.jetir.org/papers/JETIRFW06051.pdf

"In-Vitro Inhibitory Effect of Standard Phenolic Compounds against Human Salivary Metalloproteinases", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 4, page no. pp385-393, April-2023, Available at : http://www.jetir.org/papers/JETIRFW06051.pdf