• Madhuri Lele
• Shweta Patel

A significant protease that was identified from pineapple (Ananas comosus) is called bromelain. Depending on its source, bromelain accumulates to a different degree and has varied qualities throughout the entire plant. Using sodium citrate buffer and Whatman filter paper, bromelain was recovered from the entire pineapple. For 10 minutes, the filtrate was centrifuged at 6000 rpm. This was regarded as a crude enzyme. The aforementioned content underwent a step of dialysis to produce the purified enzyme. A gelatin digestion unit was developed to assess and visualize the crude extract's activity in the hydrolysis of gelatin (GDU). BSA was used as a standard in the Folin-Lowry assay, which was used to estimate the protein concentration. On the basis of various pH, temperature, activator, and inhibitor values, the enzyme's kinetics were estimated. This study's objective was to assess if bromelain, a substance extracted from pineapple waste, could reduce acne because of its numerous antibacterial qualities.

Protease, Activator, Inhibitor, Antioxidant, Antibacterial

"EXTRACELLULAR PRODUCTION AND MICROBIAL IDENTIFICATION OF BROMELIAN ENZYME FROM PINEAPPLE (ANANAS COMOSUS)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 6, page no.77-83, June-2023, Available :http://www.jetir.org/papers/JETIRFY06011.pdf

"EXTRACELLULAR PRODUCTION AND MICROBIAL IDENTIFICATION OF BROMELIAN ENZYME FROM PINEAPPLE (ANANAS COMOSUS)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 6, page no. pp77-83, June-2023, Available at : http://www.jetir.org/papers/JETIRFY06011.pdf