• Sawant R.
• Kamat S.
• Salvi P
• Vagare R
• Dalvi R

The tissue culture involves the growth of tissues or cells in an artificial medium. It is generally performed on MS medium and gives more plantlets in a short time. This medium is however prone to microbial contamination therefore, modification in MS medium is needed. Current research highlights the use of FeO and CuO NPs at two different concentrations (5 and 50 mM) in the MS media to check the effect of Nps on plant growth promotion as well as prevention of Microbial contamination. The modified MS medium was used to check its effect on seed germination, micropropagation and callus induction. This study showed the effect of CuO and FeO Nps on plant growth using Tissue culture techniques. UV spectroscopy, FITR, and FEG-SEM techniques were used to check the qualitative and quantitative nature of nanoparticles. The modified MS medium supplemented with suitable Np concentration was used to check its effect on seed germination, micropropagation, and callus culture. Out of two nanoparticles FeO Nps showed better result for seed germination whereas CuO Nps were found to be best for plant micropropagation. Supplementation of only Nps were not found to be suitable for Callus induction in given plant. Cu and Fe are vital trace elements for plant growth and they play important role in plant metabolism. Effect of these ions on plant growth as well as their importance to control microbial contamination was confirmed by modifying MS medium. There was an increase in growth percentage was observed. This finding is very important as higher number of plantlets can be obtained as well as microbial contamination also can be prevented. The present investigation also promises reduction in problems which can arise due to depletion of fertile soil and its inconvenience.

Modified MS medium, CuO NPs, FeO NPs, micropropagation.

"EFFECT OF CuO AND FeO NANOPARTICLES ON SEED GERMINATION, MICROPROPAGATION, AND CALLUS CULTURE.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 6, page no.160-169, June-2023, Available :http://www.jetir.org/papers/JETIRFY06020.pdf

"EFFECT OF CuO AND FeO NANOPARTICLES ON SEED GERMINATION, MICROPROPAGATION, AND CALLUS CULTURE.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 6, page no. pp160-169, June-2023, Available at : http://www.jetir.org/papers/JETIRFY06020.pdf