• Gurunath Pandit
• Shailendra Dalvi
• Gunesh Gundi
• Shivani Gaonkar

This paper describes a simple, accurate and precise HPTLC method for determination and validation of Warfarin Sodium Clathrate in tablet dosage form. During optimization of Mobile phase different solvents viz. n-Hexane, methanol and acetic acid were tried, however peak shape of Warfarin Sodium Clathrate was not proper. A mobile phase of Toluene : Acetone : Methanol (7 : 2 : 1 v/v) was developed for the densitometric determination of the drug product. Aluminum plate coated with the Silica Gel 60 F254 was used as stationary phase. UV spectra were taken in the range of 200 nm to 400 nm. Densitometric evaluation of the separated band of Warfarin Sodium Clathrate was performed at 306 nm. The Rf value of Warfarin Sodium Clathrate was 0.65. Developed method was linear over the range of 12.50 μg/ml to 37.50 μg/ml. Precision of the method was evaluated by calculating RSD for peak response by inter-day and intraday analysis. Accuracy was determined in terms of percentage recovery at three concentration levels for Warfarin Sodium Clathrate. The results for Warfarin Sodium Clathrate at three concentration levels are 101.00%, 101.10% and 100.60%. There was no any interference of methanol being used as a diluent and placebo at the Rf values of Warfarin Sodium Clathrate. Standard spectrum and sample spectrum of Warfarin Sodium Clathrate were overlain to confirm Rf values. ICH guidelines were referred for validation of an optimized method.

Warfarin Sodium Clathrate, Anticoagulants, High Performance thin layer chromatography, tablet dosage.

"QUANTIFICATION OF WARFARIN SODIUM CLATHRATE USING HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY FROM BULK DRUG AND PHARMACEUTICAL DOSAGE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 6, page no.186-192, June-2023, Available :http://www.jetir.org/papers/JETIRFY06023.pdf

"QUANTIFICATION OF WARFARIN SODIUM CLATHRATE USING HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY FROM BULK DRUG AND PHARMACEUTICAL DOSAGE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 6, page no. pp186-192, June-2023, Available at : http://www.jetir.org/papers/JETIRFY06023.pdf