Abstract
Grapes are recognized as one of the most valuable fruits in worldwide, approximately 60 million tonnes production form China, USA, Turkey and Italy in a year (Ma & Zhang, 2017).China emerging as the predominant cultivator by 2024.India is seventh largest producer in the world. The primary cultivators in India include Maharashtra (80%) (Nasik, Solapur, Sangli, and Pune districts), followed by northern Karnataka (Bijapur, Belgaum, Kadappa, Bagalkot districts), Andhra Pradesh (Rangareddy district), and Tamil Nadu (Krishnagiri, Theni and Coimbatore districts). It grown in sub-tropical climate where temperature range between (25-30)ºc (Mitra, 2018) boast an 8,000-year legacy, predominantly utilized for winemaking and as dried fruit (Inglis, 2022). The 10-12 kg of seeds was present in 100 kg in the fruit (Ma & Zhang, 2017; Shivananda Nayak et al., 2011). Nowadays, seeds are not used for germination and wine industries considered as a natural waste product .They has served potent source of nutrients, particularly their seeds, which contain lower quanity of an oil present in the inner layer of endosperm and embryo, accounting for approximately 8.76% of the composition. The World Health Organization (WHO) record show that 80% of the global population following the natural remedies for health issues (Manjamalai & Grace, 2012). Traditional medicine, also referred to as indigenous or folk medicine, encapsulates accumulated medical knowledge and practices developed across diverse cultures over generations. This form of medicine employs a broad spectrum of health practices, beliefs, and therapies, including plant, animal, and mineral-based medicines, spiritual therapies, manual techniques, and exercises, applied either individually or in combination to promote well-being, as well as to treat, diagnose, or prevent illnesses. Predominantly, plants and their oils serve as natural therapies (Eshete & Molla, 2021; Karunamoorthi et al., 2012; Mafimisebi & Oguntade, 2010; Oreagba et al., 2011). The oil extracted from plants in non-conventional method , known as “Cold press oil”, has derived