• Harshadaa Joshi
• Sangeeta Sinha

Drosophila melanogaster is an excellent model organism to evaluate lethal concentration and the effect of different chemical or bioactive substances due to its short generation time and lifespan. Drosophila is more resistant to oxidative stresses and has a slight but significant increase in their mean lifespan. Boric acid, or sassolite is generally used as insecticide and has proven to be fairly lethal to many pests attacking the food materials and causing various diseases.. Thus, resistance to oxidative stress and lifespan of Drosophila can be manipulated by molecular genetic intervention. Protein X, a hydrolyzed protein based supplement is aimed to make your body stronger, improving stamina and immune system of the body. In present investigation, Drosophila was exposed to different concentrations of Boric acid and Protein X. The present study reveals that prolonged exposure to boric acid at higher concentration causes toxicity which in turn affects the hatching and life cycle of drosophila. The study reveals that the supplementation of Protein X at different concentration increases the resistance ability against oxidative stress generated by boric acid. It acts a cyoprotective molecule thereby significantly normalizing the levels of antioxidant enzymes such as catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase generated by boric acid in drosophila. Also, it helps to maintain the GSH levels in drosophila both larval and adult forms. Thus, Protein X is one of accessible source of antioxidant in pharmaceutical industries and beneficial use in targeted drugs for lowering stress related molecules in organisms. To our knowledge this is the first report on Protein X cyoprotective activity against boric Acid insult.

Protein X, Antioxidant enzymes, LC50, Gluathione, oxidative stress

"TO INVESTIGATE CYTOPROTECTIVE EFFECT OF PROTEIN X ON DROSOPHILA AGAINST BORIC ACID INSULT", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.215-223, December-2018, Available :http://www.jetir.org/papers/JETIRS006033.pdf

"TO INVESTIGATE CYTOPROTECTIVE EFFECT OF PROTEIN X ON DROSOPHILA AGAINST BORIC ACID INSULT", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp215-223, December-2018, Available at : http://www.jetir.org/papers/JETIRS006033.pdf