• J.K. Priya darshini
• DR. SHABNA ROUPAL MORRIES
• R. JASMIN SAJINI

Analytical methodologies for the determination of cyclosporin have evolved significantly over the years, providing clinicians and researchers with powerful tools for therapeutic drug monitoring and pharmacokinetic studies. Among the various techniques available, High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS) stand out as the most reliable and widely used methods. Other techniques, such as Gas Chromatography (GC), Enzyme-Linked Immunosorbent Assay (ELISA), Capillary Electrophoresis (CE), and Tandem Mass Spectrometry (MS/MS), also have their unique advantages and applications for cyclosporin determination. However, HPLC and LC-MS remain at the forefront due to their robustness, sensitivity, and ability to analyze a wide range of cyclosporin concentrations accurately. Advancements in instrumentation and software have further improved the precision and efficiency of these methodologies. Researchers and clinicians now have access to faster analysis times, reduced sample volumes, and improved data processing capabilities. Additionally, ongoing research continues to explore new sample preparation techniques and the development of novel stationary phases and mobile phases to enhance sensitivity and specificity. Overall, analytical methodologies for cyclosporin determination have come a long way, playing a crucial role in optimizing therapy, improving patient outcomes, and advancing our understanding of cyclosporin pharmacokinetics and pharmacodynamics. As technology continues to evolve, these methodologies will undoubtedly remain essential tools in clinical and research settings. This review explains several published methodologies and the instrumental conditions, which have been applied to quantify the cyclosporine within the last decade.

Cyclosporin, accuracy, sensitivity

"BEST ANALYTICAL APPROACHES FOR QUANTIFICATION OF CYCLOSPORIN IN HUMAN WHOLE BLOOD -A REVIEW FOCUSED ON HPLC & LC-MS METHODS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 7, page no.830-840, July-2024, Available :http://www.jetir.org/papers/JETIRTHE2138.pdf

"BEST ANALYTICAL APPROACHES FOR QUANTIFICATION OF CYCLOSPORIN IN HUMAN WHOLE BLOOD -A REVIEW FOCUSED ON HPLC & LC-MS METHODS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 7, page no. pp830-840, July-2024, Available at : http://www.jetir.org/papers/JETIRTHE2138.pdf