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Published in:

Volume 7 Issue 6
June-2020
eISSN: 2349-5162

Unique Identifier

JETIR2006203

Page Number

1382-1394

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Title

Production and analysis of melanin pigment from bacteria isolated from farm soil

ISSN

2349-5162

Cite This Article

"Production and analysis of melanin pigment from bacteria isolated from farm soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 6, page no.1382-1394, June-2020, Available :http://www.jetir.org/papers/JETIR2006203.pdf

Abstract

The aim of this research was production and analysis of melanin pigment producing isolate from farm soil sample and study of its photo-protective role. A pigment producing isolate was screened on Tyrosine casein media from the soil samples collected from various farms. The bacteria were identified as Pseudomonas aeruginosa by molecular characterization. Production of pigment was carried out by Submerged fermentation (SF) technique. Optimization parameters like Carbon and Nitrogen sources, along with effect of incubation time on pigment production was also studied. Maximum production was observed at 192nd hour to the incubation and pigment production was observed in Starch and L-tyrosine containing medium. The pigment extracted was found to be water soluble and was confirmed to be photo-protective using UV-Vis spectroscopic studies. The pigment showed reducing power capacity (Oyaizu 1986) which serves as potent antioxidants. Fourier Transform Infra-red spectrum (FTIR) analysis confirmed the crude melanin extract obtained as melanin.

Key Words

Melanin, L-tyrosine, Photo-protective, Antioxidants

Cite This Article

"Production and analysis of melanin pigment from bacteria isolated from farm soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 6, page no. pp1382-1394, June-2020, Available at : http://www.jetir.org/papers/JETIR2006203.pdf

Publication Details

Published Paper ID: JETIR2006203
Registration ID: 234212
Published In: Volume 7 | Issue 6 | Year June-2020
DOI (Digital Object Identifier): http://doi.one/10.1729/Journal.23778
Page No: 1382-1394
ISSN Number: 2349-5162

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Cite This Article

"Production and analysis of melanin pigment from bacteria isolated from farm soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 6, page no. pp1382-1394, June-2020, Available at : http://www.jetir.org/papers/JETIR2006203.pdf




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