• Aprajita Chauhan

Water Hyacinth Biomass (WHB) is an ideal feedstock for bioethanol production as it is rich in hemicelluloses and cellulose while the lignin content is low. This feedstock is therefore, a high source of reducing sugars (C5 and C6). Water hyacinth plants were collected from five sites selected along the river Yamuna in Delhi: A. Okhla West, B. Okhla East, C. Okhla Barrage, D. Najafgarh Drain and E. DND Flyover. As feedstock plants growing in different conditions of water quality in Delhi exhibited different sugar content, a comparative study reveals that plants from site A (Okhla West) possessed maximum reducing sugar content. The sugar content in plants growing in different sites was: A>B>C>D>E Maximum saccharification (sugar extraction) was obtained after 3% sulphuric acid pretreatment for 1.5h at 1210C. Optimization of acid pretreatment helps in breaking strong linkages between cellulose, hemicellulose and lignin, thereby making the feedstock more accessible for further enzymatic hydrolysis. Of the three yeast strains used, the Four Seasons Yeast(FSY) gave best results for bioethanol fermentation (0.198g/L) when used on 3% sugar hydrolysate from 1:10 WHB after 24h. Corresponding values of bioethanol production by other two yeasts were slightly lower. Studies in time course utilization of hydrolysate corroborated the results for efficiency of yeasts in the order: FSY> Baker’s Yeast> Yeast Extract.

Pontederia crassipes (Water Hyacinth), bio feedstock, yeast strains, bioethanol ferementation.

"Comparative Study of Three Yeast Strains on Bioethanol Fermentation of Pontederia crassipes(Water Hyacinth) Feedstock", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 2, page no.b415-b422, February-2025, Available :http://www.jetir.org/papers/JETIR2502140.pdf

"Comparative Study of Three Yeast Strains on Bioethanol Fermentation of Pontederia crassipes(Water Hyacinth) Feedstock", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 2, page no. ppb415-b422, February-2025, Available at : http://www.jetir.org/papers/JETIR2502140.pdf